ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2014, Vol. 45 ›› Issue (10): 1718-1725.doi: 10.11843/j.issn.0366-6964.2014.10.019

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Isolation and PCR-DGGE Studies of the Fungal Pathogens from Pastures with High Incidence of Sheep Facial Eczema on the Northern Slope of Tianshan Mountains

LIU Liang-bo,ZHANG Yu,SUN Zhi-hua,OU Ke-peng,SUN Huan-lin,YAN Gen-qiang*   

  1. (College of Animal Science & Technology,Shihezi University,Shihezi 832003,China)
  • Received:2014-02-07 Online:2014-10-23 Published:2014-10-23

Abstract:

This paper was undertaken to investigate fungi species which cause facial eczema (FE) of grazing sheep from pastures with high incidence of FE on the northern slope of Tianshan Mountains,and analyze their diversity,then identify the relationship among disease occurrence grazing location,and community distribution of fungal correlation.After investigating the features of geographical environment in the FE incidence area,forage and soil samples were cultured from incidence areas and non epidemic areas during FE season.Fungi were isolated and cultured for further studies,such as morphological observation,species and distribution analysis.Total fungal DNAs were extracted,and then PCR,denaturing gradient gel electrophoresis (DGGE) and DNA sequencing of 18S rDNA were used to explore fungal diversity.The results showed that the FE incidence area located at inclined plains of piedmont alluvial fan,N44.016-44.022,E85.792-85.800,and altitude of 925-1 039 m,where expressing typical temperate continental arid climate.They are important local spring- and autumn pastures.Fungal cultivation,PCR-DGGE and sequencing analysis of 18S rDNA showed that there found 10 kinds of fungi in above areas.Separation rates of AlternariaPithomyces chartarumFusariumAspergillusPenicillium were higher,and that of Pithomyces chartarum was the highest,these results were identical with the isolated fungi in digestive tract of FE sheep.Our research indicated that Alternaria spp.,pithomyces chartarum spp., Fusarium and aspergillus maybe the responsible pathogens of hepatogenic light allergic FE.There were the correlation among grazing location,fungi distribution and FE incidence,which provides a basis for revealing the pathogenesis and the influence factors of FE in grazing sheep.

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